bbin宝盈集团

GENERAL INFORMATION
Gene Name Synonym:	coronavirus spike; cov spike; ncov RBD; ncov s1; ncov s2; ncov spike; NCP-CoV RBD; NCP-CoV s1; NCP-CoV s2; NCP-CoV Spike; novel coronavirus RBD; novel coronavirus s1; novel coronavirus s2; novel coronavirus spike; RBD; S1; S2; Spike RBD
Protein Construction:	A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit) (YP_009724390.1) (Val16-Arg685) was expressed with a polyhistidine tag at the C-terminus.
Source:	2019-nCoV
Expression Host:	Baculovirus-Insect Cells
QC Testing
Purity:	＞90 % as determined by SDS-PAGE
Bio Activity：	Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 10μg/mL (100μL/well) can bind 2019-nCoV Spike Protein (S1, His tag) (40591- V08B1), the EC50 of 2019-nCoV Spike Protein (S1, His tag) (40591- V08B1) is 300-600 ng/mL.
Endotoxin:	＜1.0 EU per μg protein as determined by the LAL method.
Predicted N terminal:	Val 16
Molecular Mass:	The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, His tag) consists of 681 amino acids and predicts a molecular mass of 76.45 kDa
Formulation:	Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
Usage Guide
Stability & Storage:	Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.
Avoid repeated freeze-thaw cycles.
Reconstitution:	Detailed reconstitution instructions are sent along with the products.

SDS-PAGE:

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